LOCUS       40924_23819       12882 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB742 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12882)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1894
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1904..2028
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2065..2095
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2149..2805
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3150..3452
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3496..3620)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3646..3898
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3932..3948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3956..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3980..4010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4025..4046)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4082..4334)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4372..5667)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61261.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4372..5667)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5719..5898)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6404..6428)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6949..7737
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7986..8574
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8760..8900)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9244..9438)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9507..10577)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11009..11635)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1381 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caaggctgtt atcacaagtt tgtacaaaaa
     1921 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1981 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
     2041 ggggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2101 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2161 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2221 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2281 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2341 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2401 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2461 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2521 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2581 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2641 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2701 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     2761 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     2821 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2881 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     2941 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3001 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3061 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3121 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3181 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3241 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3301 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3361 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3421 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3481 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3541 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3601 gctttcttgt acaaagtggt gataacctag agctcgaatt tccccgatcg ttcaaacatt
     3661 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3721 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3781 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3841 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3901 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4021 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tattaattcc
     4081 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     4141 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     4201 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     4261 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     4321 atgtttgaac gatcggggaa attcgcctcg agtgacttct ctgattgcag actatttata
     4381 ccaaccagca agaaagtgtc gaagtatgaa gcagaaggcg caggctagag cctggaaaac
     4441 gagaagatgg atgcaaagag acttttctga tttaggacca aacaacctca agtaaacatt
     4501 gacgagtgtc ccatcttttg tgccggtcag taatccggta gcaggatcat accttgggag
     4561 acgatggcgc ggacatttca caatcccagc aagctgagga agcgataata gaaggttcag
     4621 aacttgagga agaaagaaga tgaggagggt ttcactgaag tgacccagaa taccaacaac
     4681 tgccatagtc attccggcga agactgtgta agtgtctcca acaaaaactg aagaaggata
     4741 ccaattgtaa gcaagcattg ccaaggatgt ggccatcagc ggttgagtaa gaaatatcga
     4801 gaaggcatga gcttggtgat actcgggatc aacagatgct ccgatttgca taacgttgtg
     4861 tatcaggata gcagcggcaa taacaactgt ttgaccgatt tcaaggccat tcagaccagc
     4921 gtggatgtta atagagtttg tacaaaagac agcaagtagc cccatatata acttatatat
     4981 ccttcctaga ttaagcactt ccaagccaat ataagcaaca agaggttttg gtataacaat
     5041 agttgtatga ccagcataag ccatcaaaag tggaagggta gcaaaagatg gcaggacaag
     5101 ttttactctc caaggcacat caaggacatc atctacaaat ccaagcaaaa tcatgaagca
     5161 aatagatgct agtgctgcat tatactccac aagccaatta gaatcttcag tgaagttaaa
     5221 atactgaaat attatcgcca cgatcaaaaa gacaatacca acaacaatac ccaatgactc
     5281 tggcacttta atatctcctt gaggcgtgcc tcttttgttg atatcaaaac caaacatgtt
     5341 acgtctcaga acgtatctgg cagtaacagg aatcattttg agagtgacga aaaacccaac
     5401 caagctaagc cccgcgttaa ttagaatcga tcgcttgagt tcattatcaa ccttgtagtg
     5461 aaaacagaga aggtatagat agagcgaaca gagaagagta gagatcacga agatcactcc
     5521 taatttcggc ggcgcgagac gaaattcctc accggaagca gaaaccgtct ttcgggtcat
     5581 cttctgctcc gatggcgctg agttgggttc cgacgggtcc ggtttgttgg gaattgagat
     5641 cgatgaagct ctcttacgag ctgccatatc ttattgcccc ccgggatcag atctggattg
     5701 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
     5761 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
     5821 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct
     5881 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
     5941 gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc tgcgccatca
     6001 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     6061 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     6121 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     6181 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     6241 ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaagc
     6301 tgatccgtcg agattaatag aaataaattc agcctaattc ggcgttaatt cagtacatta
     6361 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     6421 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     6481 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     6541 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     6601 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     6661 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     6721 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     6781 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     6841 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     6901 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     6961 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     7021 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     7081 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     7141 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     7201 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     7261 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     7321 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     7381 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     7441 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     7501 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     7561 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     7621 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     7681 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     7741 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     7801 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     7861 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     7921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8101 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     8161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8761 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     8821 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     8881 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8941 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9001 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     9061 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     9121 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     9181 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     9241 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     9301 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     9361 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     9421 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     9481 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     9541 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     9601 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     9661 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     9721 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     9781 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     9841 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     9901 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     9961 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
    10021 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
    10081 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
    10141 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
    10201 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    10261 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    10321 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    10381 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    10441 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    10501 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    10561 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    10621 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    10681 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    10741 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    10801 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    10861 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    10921 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    10981 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    11041 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    11101 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    11161 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    11221 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    11281 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    11341 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    11401 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    11461 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    11521 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    11581 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    11641 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    11701 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    11761 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    11821 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    11881 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    11941 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    12001 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    12061 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    12121 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    12181 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    12241 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    12301 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    12361 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    12421 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    12481 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    12541 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    12601 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    12661 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    12721 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    12781 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    12841 tgtggttggc atgcacatac aaatggacga acggataaac ct
//
